ABSTRACT
Background: Colorectal cancer (CRC) is currently the third most common cancer type in males and the second most occurring in females. The role of microRNA (miRNA) in the development of colorectal cancer is notfully elucidated. Therefore, understanding the mechanistic interaction between miRNA and their target oncogenes may hold great importance as a possible target for interventional anticancer therapy Aims:To identify miRNAs that are part of the regulating pathway of Monocarboxylate Transporter-4 (MCT4) and Vascular Endothelial Growth Factor (VEGF) oncogenes.Study Design:We used publicly available prediction tools (e.g. TargetScan, MicroCosm, PicTar, and DIANA-microT-CDS) to identify the possible miRNA that target the two oncogenes. Methodology:We used the GeneMania database to visualize the network and verify gene names and remove ambiguity and duplications. Furthermore, we used miRTarBase database to identify experimentally validated targets which we used to further confirm miRNA-oncogene relationships. Finally, we utilized miR-Mfold web-tool to further visualize the circular structures and the simulated miR-1 and miR-206 targeting arrangements.Results:We found two putative miRNA (miR-1 and miR-206) that may downregulate MCT4 coded by SLC16A3gene and VEGF which is coded by VEGF gene. We found relationships between the validated target genes of miR-1 and miR-206 through GeneMania which we extracted from the literature. And we elucidated the proposed structure of these two miRNAs through miR-Mfold web-tool.Conclusion: Our results elucidated a novel regulation pathway in CRC cells and may suggest a potential therapeutic approach for CRC therapy. MiR-1 and miR-206 may help cells go to apoptosis and inhibit the angiogenesis of colorectal cancer cells by down-regulation of MCT4 and VEGF proteins in tumor tissues.
ABSTRACT
Aims: To evaluate the associations between bedtime initiations, sleeping sufficiency and objective academic performance in a large sample of second secondary students.Methods: Thestudy sample consisted of 900 adolescents; 450boysand 450 girls in the second secondary class at Hail city in the Kingdom of Saudi Arabia.Participants filled a questioner about their socioeconomic factors as bedtime, sleeping duration, mental games practicing, and physical activity.Weight and heightwere measured to assess body mass index (kg/m2). Data extracted from the questioner used to study the contribution of the concomitant factors on academic achievement.Results: The mean weight was 73.57±8.34kg IQR (44-126) while the mean height was (170.38±6.45) cm IQR (141-192). The mean BMI was 33.57±14.79(IQR 21.0–38.8). The overall prevalence of childhood obesity was 460 (49.6%; 95% CI: 55.3–66.8%) while the prevalence of overweight was 85 (9.04%; 95%CI: 7.3 –11.4%). The mean ministry academic score for the second secondary class was 70±9.99 IQR (44-99) while the school academic score was 88.07±7.44 IQR (60-99).51.6% of the students went to bed between 10-12 pm and only 17.6% slept between 8 and 10 hours.Conclusion: We can summarize, the independent variables as bedtime, sleeping duration, and obesity ameliorate academic achievement in a counteractive way; high BMI above 35 and prolonged sleeping duration more than 10 hours significantly curb the total academic score, whereas, healthy bedtime of 8-10 pm concomitantly augment the academic achievement